Application

Western blot: 1-10 µg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000 against the control peptide (Catalog Number AG794).

Optimal working dilutions must be determined by end user.

Research CategoryNeuroscience

Detect Orexin-2 Receptor using this Anti-Orexin-2 Receptor Antibody validated for use in ELISA, IH & WB.

Research Sub CategoryCNS Control of MetabolismCircadian Rhythm & Sleep

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 19 aa peptide near the C-terminus (cytoplasmic domain) of rat OX2R. The immunogen peptide (Catalog Number AG794) is available to perform preabsorbtion controls.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified 1mg/mL solution in PBS, pH 7.4 and 0.1% BSA

Specificity

Rat/Human Orexin-2. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.